diff --git a/src/detect/ScanI2CTwoWire.cpp b/src/detect/ScanI2CTwoWire.cpp
index f09eb3b95..472a9d70a 100644
--- a/src/detect/ScanI2CTwoWire.cpp
+++ b/src/detect/ScanI2CTwoWire.cpp
@@ -162,13 +162,13 @@ void ScanI2CTwoWire::scanPort(I2CPort port, uint8_t *address, uint8_t asize)
     Melopero_RV3028 rtc;
 #endif
 
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
     if (port == I2CPort::WIRE1) {
         i2cBus = &Wire1;
     } else {
 #endif
         i2cBus = &Wire;
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
     }
 #endif
 
@@ -423,7 +423,7 @@ TwoWire *ScanI2CTwoWire::fetchI2CBus(ScanI2C::DeviceAddress address) const
     if (address.port == ScanI2C::I2CPort::WIRE) {
         return &Wire;
     } else {
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
         return &Wire1;
 #else
         return &Wire;
diff --git a/src/gps/RTC.cpp b/src/gps/RTC.cpp
index 728284242..98dea4674 100644
--- a/src/gps/RTC.cpp
+++ b/src/gps/RTC.cpp
@@ -29,7 +29,7 @@ void readFromRTC()
     if (rtc_found.address == RV3028_RTC) {
         uint32_t now = millis();
         Melopero_RV3028 rtc;
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
         rtc.initI2C(rtc_found.port == ScanI2C::I2CPort::WIRE1 ? Wire1 : Wire);
 #else
         rtc.initI2C();
@@ -58,7 +58,7 @@ void readFromRTC()
         uint32_t now = millis();
         PCF8563_Class rtc;
 
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
         rtc.begin(rtc_found.port == ScanI2C::I2CPort::WIRE1 ? Wire1 : Wire);
 #else
         rtc.begin();
@@ -150,7 +150,7 @@ bool perhapsSetRTC(RTCQuality q, const struct timeval *tv, bool forceUpdate)
 #ifdef RV3028_RTC
         if (rtc_found.address == RV3028_RTC) {
             Melopero_RV3028 rtc;
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
             rtc.initI2C(rtc_found.port == ScanI2C::I2CPort::WIRE1 ? Wire1 : Wire);
 #else
             rtc.initI2C();
@@ -164,7 +164,7 @@ bool perhapsSetRTC(RTCQuality q, const struct timeval *tv, bool forceUpdate)
         if (rtc_found.address == PCF8563_RTC) {
             PCF8563_Class rtc;
 
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
             rtc.begin(rtc_found.port == ScanI2C::I2CPort::WIRE1 ? Wire1 : Wire);
 #else
             rtc.begin();
diff --git a/src/input/cardKbI2cImpl.cpp b/src/input/cardKbI2cImpl.cpp
index 8aaebcb45..1f7fd284d 100644
--- a/src/input/cardKbI2cImpl.cpp
+++ b/src/input/cardKbI2cImpl.cpp
@@ -16,7 +16,7 @@ void CardKbI2cImpl::init()
         uint8_t i2caddr_asize = 3;
         auto i2cScanner = std::unique_ptr<ScanI2CTwoWire>(new ScanI2CTwoWire());
 
-#if defined(I2C_SDA1)
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
         i2cScanner->scanPort(ScanI2C::I2CPort::WIRE1, i2caddr_scan, i2caddr_asize);
 #endif
         i2cScanner->scanPort(ScanI2C::I2CPort::WIRE, i2caddr_scan, i2caddr_asize);
diff --git a/src/input/kbI2cBase.cpp b/src/input/kbI2cBase.cpp
index 2692fc80d..a25a85d82 100644
--- a/src/input/kbI2cBase.cpp
+++ b/src/input/kbI2cBase.cpp
@@ -33,7 +33,7 @@ int32_t KbI2cBase::runOnce()
     if (!i2cBus) {
         switch (cardkb_found.port) {
         case ScanI2C::WIRE1:
-#ifdef I2C_SDA1
+#if defined(I2C_SDA1) || (defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2))
             LOG_DEBUG("Using I2C Bus 1 (the second one)\n");
             i2cBus = &Wire1;
             if (cardkb_found.address == BBQ10_KB_ADDR) {
diff --git a/src/main.cpp b/src/main.cpp
index e24ba68b3..0ab9c0e34 100644
--- a/src/main.cpp
+++ b/src/main.cpp
@@ -360,6 +360,8 @@ void setup()
     Wire1.begin();
 #elif defined(I2C_SDA1) && !defined(ARCH_RP2040)
     Wire1.begin(I2C_SDA1, I2C_SCL1);
+#elif defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2)
+    Wire1.begin();
 #endif
 
 #if defined(I2C_SDA) && defined(ARCH_RP2040)
@@ -427,6 +429,8 @@ void setup()
 #elif defined(I2C_SDA1) && !defined(ARCH_RP2040)
     Wire1.begin(I2C_SDA1, I2C_SCL1);
     i2cScanner->scanPort(ScanI2C::I2CPort::WIRE1);
+#elif defined(NRF52840_XXAA) && (WIRE_INTERFACES_COUNT == 2)
+    i2cScanner->scanPort(ScanI2C::I2CPort::WIRE1);
 #endif
 
 #if defined(I2C_SDA) && defined(ARCH_RP2040)